Recombinant Mouse Guanine nucleotide-binding protein G (o) subunit alpha (Gnao1), Mus musculus (Mouse), Yeast, N-terminal 6xHis-tagged, 42.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Guanine nucleotide-binding protein G (o) subunit alpha (Gnao1)

Recombinant Mouse Guanine nucleotide-binding protein G (o) subunit alpha (Gnao1) — Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. Purity >90%.

SKU: BCRECP-002880 Category:
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Product Specifications

Product SkuBCRECP-002880
Product DescriptionRecombinant Mouse Guanine nucleotide-binding protein G (o) subunit alpha (Gnao1) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 2-354aa. [Accession | P18872].
Uniprot No.P18872
Gene NamesGnao1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-354aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight42.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding.

Biological Context

Subcellular Location: Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Mouse Guanine nucleotide-binding protein G (o) subunit alpha (Gnao1) is a recombinant protein from Mus musculus (Mouse), expressed in Yeast, covering amino acids 2-354aa, with N-terminal 6xHis-tagged tag, molecular weight 42.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:10926822). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:10926822). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:10926822). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:10926822). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:10926822). Signaling is mediated via effector proteins, such as adenylate cyclase (PubMed:10926822). Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:10926822)

Subcellular Location

Cell membrane (Peripheral membrane protein)

Subunit

G proteins are composed of 3 units; alpha, beta and gamma (By similarity). The alpha chain contains the guanine nucleotide binding site (By similarity). Forms a complex with GNB1 and GNG3 (By similarity). Forms a complex with GNB1 and GNG2; this complex associates with Mu-type opioid receptor OPRM1, D(2) dopamine receptor DRD2, neurotensin receptor type 1 NTSR1 and beta-2 adrenergic receptor ADRB2 (By similarity). Interacts with RGS14 (PubMed:10926822). Interacts with RGS16 (PubMed:18434540). Interacts with RGS19 (By similarity). Interacts (when palmitoylated) with ADGRG3 (By similarity)

Gene: Gnao1  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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