Recombinant Mouse Interferon regulatory factor 1 (Irf1), Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 44.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse Interferon regulatory factor 1 (Irf1)

Recombinant Mouse Interferon regulatory factor 1 (Irf1) — Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Purity >90%.

SKU: BCRECP-003186 Categories: ,
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Product Specifications

Product SkuBCRECP-003186
Product DescriptionRecombinant Mouse Interferon regulatory factor 1 (Irf1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-329aa. [Accession | P15314].
Uniprot No.P15314
Gene NamesIrf1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-329aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight44.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters.

Biological Context

Subcellular Location: Nucleus; Cytoplasm

Product Specifications

Recombinant Mouse Interferon regulatory factor 1 (Irf1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-329aa, with C-terminal 6xHis-tagged tag, molecular weight 44.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750, PubMed:20049431, PubMed:25774715). Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750, PubMed:20049431, PubMed:2208287, PubMed:3409321, PubMed:8402903). Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750, PubMed:20049431). Has an essential role in IFNG-dependent immunity to mycobacteria (By similarity). Binds to a consensus sequence in gene promoters (By similarity). Its target genes for transcriptional activation activity are: genes involved in anti-viral response, such as IFN-alpha/beta, RIGI, TNFSF10/TRAIL, ZBP1, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as GBP2, GBP5, IRGB10 and NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA; metabolic enzymes, such as ACOD1/IRG1 (PubMed:12387893, PubMed:17018642, PubMed:18955028, PubMed:20308629, PubMed:21909274, PubMed:25774715, PubMed:27693356, PubMed:29321274, PubMed:30635240). Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4 (PubMed:18641303). Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53 (By similarity). Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750, PubMed:20049431). Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750, PubMed:20049431). Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750, PubMed:20049431)

Subcellular Location

Nucleus; Cytoplasm

Subunit

Monomer (PubMed:9422515). Homodimer (PubMed:9422515). Interacts with EP300 (By similarity). Interacts with MYD88 (PubMed:17018642). Interacts with PIAS3 (PubMed:12387893). Interacts with SPOP (By similarity)

Gene: Irf1  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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