Recombinant Mouse Interferon regulatory factor 8 (Irf8), Mus musculus (Mouse), E.coli, N-terminal 6xHis-SUMO-tagged, 52.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse Interferon regulatory factor 8 (Irf8)

Recombinant Mouse Interferon regulatory factor 8 (Irf8) — Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Purity >90%.

SKU: BCRECP-003187 Categories: ,
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Product Specifications

Product SkuBCRECP-003187
Product DescriptionRecombinant Mouse Interferon regulatory factor 8 (Irf8) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-424aa. [Accession | P23611].
Uniprot No.P23611
Gene NamesIrf8
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-424aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight52.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Can both act as a transcriptional activator or repressor. Plays a negative regulatory role in cells of the immune system.

Biological Context

Subcellular Location: Nucleus; Cytoplasm
Tissue Specificity: Expressed in bone marrow macrophages (at protein level). Mainly expressed in lymphoid tissues. Predominantly expressed in CD8(+)-expressing dendritic cells

Product Specifications

Recombinant Mouse Interferon regulatory factor 8 (Irf8) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-424aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 52.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed:12393690, PubMed:2111015). Can both act as a transcriptional activator or repressor (PubMed:2111015). Plays a negative regulatory role in cells of the immune system (PubMed:2111015). Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed:12393690, PubMed:22992524). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (PubMed:12393690, PubMed:12461077, PubMed:12538667, PubMed:23382217). Positively regulates macroautophagy in dendritic cells (By similarity). Acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed:32741026)

Subcellular Location

Nucleus; Cytoplasm

Tissue Specificity

Expressed in bone marrow macrophages (at protein level) (PubMed:32741026). Mainly expressed in lymphoid tissues (PubMed:2111015). Predominantly expressed in CD8(+)-expressing dendritic cells (PubMed:12393690)

Subunit

Interacts with COPS2 (By similarity). Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct. Interacts with SPI1 (By similarity)

Gene: Irf8  |  Organism: Mus musculus  |  Synonyms: Interferon consensus sequence-binding protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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