Recombinant Mouse Interleukin-1 beta (Il1b) (Active)

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore

Subcellular Location

Cytoplasm, cytosol; Secreted; Lysosome; Secreted, extracellular exosome

Tissue Specificity

Expressed in activated macrophages (at protein level)

Subunit

Monomer. Interacts with MEFV. Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling. Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form (By similarity). Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC (By similarity). Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC (By similarity)

• Gray P.W. et al. Two interleukin 1 genes in the mouse: cloning and expression of the cDNA for murine interleukin 1 beta. — J. Immunol. (1986)
• Telford J.L. et al. The murine interleukin 1 beta gene: structure and evolution. — Nucleic Acids Res. (1986)
• Farber C.R. et al. Characterization of quantitative trait loci influencing growth and adiposity using congenic mouse strains. (JAN-)
• Mural R.J. et al. PubMed: CI-70VOHH7BF94TO (JUL-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Huang J.J. et al. Characterization of murine IL-1 beta. Isolation, expression, and purification. — J. Immunol. (1988)
• Mariathasan S. et al. Cryopyrin activates the inflammasome in response to toxins and ATP. — Nature (2006)
• Brough D. et al. Caspase-1-dependent processing of pro-interleukin-1beta is cytosolic and precedes cell death. — J. Cell Sci. (2007)