Recombinant Mouse Interleukin-2 protein (Il2), Mus musculus (Mouse), E.Coli, Tag-Free, 17.2 kDa, purity >>95% as determined by SDS-PAGE.
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Recombinant Mouse Interleukin-2 protein (Il2)

Recombinant Mouse Interleukin-2 protein (Il2) — Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and to… Purity >95%.

SKU: BCACP-000713 Categories: ,
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Product SKUBCACP-000713
Product DescriptionRecombinant Mouse Interleukin-2 protein (Il2) protein is expressed from E.Coli with Tag-Free. It contains 21-169aa. [Accession | P04351].
Uniprot No.P04351
Gene NamesIl2
Purity>95% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region21-169aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight17.2 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of >5x107 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIl2; Il-2Interleukin-2; IL-2; T-cell growth factor; TCGF

Function

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Produced by immune cells including dendritic cells. In contrast, macrophages do not produce IL2 upon bacterial stimulation

Product Specifications

Recombinant Mouse Interleukin-2 protein (Il2) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 21-169aa, with Tag-Free tag, molecular weight 17.2 kDa, purity >95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of >5x107 IU/mg.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:14614860, PubMed:9814585). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:14614860, PubMed:27018889). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance (PubMed:14614860). Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells (PubMed:9814585)

Subcellular Location

Secreted

Tissue Specificity

Produced by immune cells including dendritic cells. In contrast, macrophages do not produce IL2 upon bacterial stimulation

Gene: Il2  |  Organism: Mus musculus  |  Synonyms: T-cell growth factor
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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