Recombinant Mouse Interleukin-5 protein (Il5), Mus musculus (Mouse), E.Coli, Tag-Free, 13.1 kDa, purity >>98% as determined by SDS-PAGE.
,

Recombinant Mouse Interleukin-5 protein (Il5)

Recombinant Mouse Interleukin-5 protein (Il5) — Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Purity >98%.

SKU: BCACP-000715 Categories: ,
Online Inquiry →
Product SKUBCACP-000715
Product DescriptionRecombinant Mouse Interleukin-5 protein (Il5) protein is expressed from E.Coli with Tag-Free. It contains 21-133aa. [Accession | P04401].
Uniprot No.P04401
Gene NamesIl5
Purity>98% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region21-133aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight13.1 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIl5; Il-5Interleukin-5; IL-5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF

Function

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in lymphoid cells, including spleen, thymus, lymph nodes and peripheral blood mononuclear cells

Product Specifications

Recombinant Mouse Interleukin-5 protein (Il5) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 21-133aa, with Tag-Free tag, molecular weight 13.1 kDa, purity >98% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:10444455, PubMed:1873482). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (PubMed:3128631). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (By similarity)

Subcellular Location

Secreted

Tissue Specificity

Expressed in lymphoid cells, including spleen, thymus, lymph nodes and peripheral blood mononuclear cells

Subunit

Homodimer; disulfide-linked. Interacts with IL5RA (PubMed:1873482). Interacts with CSF2RB

Gene: Il5  |  Organism: Mus musculus  |  Synonyms: B-cell growth factor II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top