Recombinant Mouse Interleukin-9 protein (Il9) (Active), Mus musculus (Mouse), E.Coli, Tag-Free, 14.2 kDa, purity >>97% as determined by SDS-PAGE.
,

Recombinant Mouse Interleukin-9 protein (Il9) (Active)

Recombinant Mouse Interleukin-9 protein (Il9) (Active) — Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites. Purity >97%.

SKU: BCACP-000717 Categories: ,
Online Inquiry →
Product SKUBCACP-000717
Product DescriptionRecombinant Mouse Interleukin-9 protein (Il9) protein is expressed from E.Coli with Tag-Free. It contains 19-144aa. [Accession | P15247].
Uniprot No.P15247
Gene NamesIl9
Purity>97% as determined by SDS-PAGE.
Expression SystemE.Coli
Expression Region19-144aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight14.2 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine T11 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0x105 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesIl9; Interleukin-9; IL-9; Cytokine P40; T-cell growth factor P40

Function

Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites. Affects intestinal epithelial permeability and adaptive immunity. In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Mouse Interleukin-9 protein (Il9) (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.Coli, covering amino acids 19-144aa, with Tag-Free tag, molecular weight 14.2 kDa, purity >97% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites (PubMed:11070175, PubMed:19433802). Affects intestinal epithelial permeability and adaptive immunity (PubMed:12704113). In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells (PubMed:11070175, PubMed:19433802). Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG (PubMed:2145361, PubMed:7718508). Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs (PubMed:10464327). Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5 (PubMed:10464327)

Subcellular Location

Secreted

Subunit

Interacts with IL9R (PubMed:2145361). Interacts with IL2RG (PubMed:7718508)

Gene: Il9  |  Organism: Mus musculus  |  Synonyms: Cytokine P40; T-cell growth factor P40
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top