Recombinant Mouse Isoleucine--tRNA ligase,cytoplasmic (Iars1), partial, Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 16.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Mouse Isoleucine–tRNA ligase,cytoplasmic (Iars1), partial

Recombinant Mouse Isoleucine–tRNA ligase,cytoplasmic (Iars1), partial — recombinant protein from Mus musculus (Mouse), expressed in E.coli. Purity >85%.

SKU: BCRECP-003050 Categories: ,
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Product Specifications

Product SkuBCRECP-003050
Product DescriptionRecombinant Mouse Isoleucine--tRNA ligase,cytoplasmic (Iars1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 732-831aa. [Accession | Q8BU30].
Uniprot No.Q8BU30
Gene NamesIars1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region732-831aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight16.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Biological Context

Subcellular Location: Cytoplasm; Cytoplasm, cytosol

Product Specifications

Recombinant Mouse Isoleucine–tRNA ligase,cytoplasmic (Iars1), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 732-831aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 16.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA

Subcellular Location

Cytoplasm; Cytoplasm, cytosol

Subunit

Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18

Gene: Iars1  |  Organism: Mus musculus  |  Synonyms: Isoleucyl-tRNA synthetase

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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