Recombinant Mouse L-dopachrome tautomerase (Dct), partial, Mus musculus (Mouse), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 71.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse L-dopachrome tautomerase (Dct), partial

Recombinant Mouse L-dopachrome tautomerase (Dct), partial — Plays a role in melanin biosynthesis. Purity >90%.

SKU: BCRECP-002512 Category:
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Product Specifications

Product SkuBCRECP-002512
Product DescriptionRecombinant Mouse L-dopachrome tautomerase (Dct) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 24-472aa. [Accession | P29812].
Uniprot No.P29812
Gene NamesDct
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-472aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight71.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays a role in melanin biosynthesis. Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).

Biological Context

Subcellular Location: Melanosome membrane (Single-pass type I membrane protein); Melanosome
Tissue Specificity: Melanocytes and retinal pigmented epithelium (at protein level)
Disease Association: [The slaty mutation in Tyrp2 leads to a decrease of DT activity and a consequent change in the pigmentation of the mice to a dark gray/brown eumelanin. The slaty-2j mutation has a similar phenotype, the slaty-lt (light) mutation has a more severe effect and is semidominant; its phenotype may be a result of the failure of the enzyme to be correctly targeted to its normal location on the inner face of the melanosomal membrane]
Pathway: Pigment biosynthesis; melanin biosynthesis

Product Specifications

Recombinant Mouse L-dopachrome tautomerase (Dct), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 24-472aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 71.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA) (PubMed:1537333, PubMed:1537334)

Subcellular Location

Melanosome membrane (Single-pass type I membrane protein); Melanosome

Disease Association

[The slaty mutation in Tyrp2 leads to a decrease of DT activity and a consequent change in the pigmentation of the mice to a dark gray/brown eumelanin. The slaty-2j mutation has a similar phenotype, the slaty-lt (light) mutation has a more severe effect and is semidominant; its phenotype may be a result of the failure of the enzyme to be correctly targeted to its normal location on the inner face of the melanosomal membrane]

Tissue Specificity

Melanocytes and retinal pigmented epithelium (at protein level)

Subunit

Forms an OPN3-dependent complex with TYR in response to blue light in melanocytes

Pathway

Pigment biosynthesis; melanin biosynthesis

Gene: Dct  |  Organism: Mus musculus  |  Synonyms: DOPAchrome conversion factor; DOPAchrome isomerase; DOPAchrome oxidoreductase; L-dopachrome Delta-isomerase; SLATY locus protein; Tyrosinase-related protein 2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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