Recombinant Mouse Myoglobin (Mb), Mus musculus (Mouse), Yeast, N-terminal 6xHis-tagged and C-terminal Myc-tagged, 18.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Myoglobin (Mb)

Recombinant Mouse Myoglobin (Mb) — Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Purity >90%.

SKU: BCRECP-003360 Category:
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Product Specifications

Product SkuBCRECP-003360
Product DescriptionRecombinant Mouse Myoglobin (Mb) Protein is expressed from Yeast with N-terminal 6xHis-tagged and C-terminal Myc-tagged. It contains 2-154aa. [Accession | P04247].
Uniprot No.P04247
Gene NamesMb
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-154aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged and C-terminal Myc-tagged
Molecular weight18.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand. Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide.

Biological Context

Subcellular Location: Cytoplasm, sarcoplasm

Product Specifications

Recombinant Mouse Myoglobin (Mb) is a recombinant protein from Mus musculus (Mouse), expressed in Yeast, covering amino acids 2-154aa, with N-terminal 6xHis-tagged and C-terminal Myc-tagged tag, molecular weight 18.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed:10468637, PubMed:11304494). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (By similarity). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed:15132981)

Subcellular Location

Cytoplasm, sarcoplasm

Subunit

Monomeric

Gene: Mb  |  Organism: Mus musculus  |  Synonyms: Nitrite reductase MB; Pseudoperoxidase MB
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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