Recombinant Mouse Nicotinamide phosphoribosyltransferase (Nampt) (C39S), Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged and C-terminal HA-tagged, 60.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse Nicotinamide phosphoribosyltransferase (Nampt) (C39S)

Recombinant Mouse Nicotinamide phosphoribosyltransferase (Nampt) (C39S) — The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by A… Purity >90%.

SKU: BCRECP-003844 Categories: ,
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Product Specifications

Product SkuBCRECP-003844
Product DescriptionRecombinant Mouse Nicotinamide phosphoribosyltransferase (Nampt) (C39S) Protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal HA-tagged. It contains 1-491aa(C39S). [Accession | Q99KQ4].
Uniprot No.Q99KQ4
Gene NamesNampt
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-491aa(C39S)
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged and C-terminal HA-tagged
Molecular weight60.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasBiochemicals

Function

The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.

Biological Context

Subcellular Location: Nucleus; Cytoplasm; Secreted
Tissue Specificity: Ubiquitously expressed in lymphoid and non-lymphoid tissues
Pathway: Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1

Product Specifications

Recombinant Mouse Nicotinamide phosphoribosyltransferase (Nampt) (C39S) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-491aa(C39S), with N-terminal 6xHis-tagged and C-terminal HA-tagged tag, molecular weight 60.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (By similarity). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression

Subcellular Location

Nucleus; Cytoplasm; Secreted

Tissue Specificity

Ubiquitously expressed in lymphoid and non-lymphoid tissues

Subunit

Homodimer

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1

Gene: Nampt  |  Organism: Mus musculus  |  Synonyms: Pre-B-cell colony-enhancing factor 1 homolog; Visfatin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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