Recombinant Mouse Nuclear factor interleukin-3-regulated protein (Nfil3), Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 57.0kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Mouse Nuclear factor interleukin-3-regulated protein (Nfil3)

Recombinant Mouse Nuclear factor interleukin-3-regulated protein (Nfil3) — Acts as a transcriptional regulator that recognizes and binds to the sequence 5′-[GA]TTA[CT]GTAA[CT]-3′, a sequence present in many cellular and viral promoters. Purity >85%.

SKU: BCRECP-003865 Categories: ,
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Product Specifications

Product SkuBCRECP-003865
Product DescriptionRecombinant Mouse Nuclear factor interleukin-3-regulated protein (Nfil3) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-462aa. [Accession | O08750].
Uniprot No.O08750
Gene NamesNfil3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-462aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight57.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts.

Product Specifications

Recombinant Mouse Nuclear factor interleukin-3-regulated protein (Nfil3) is a recombinant protein, expressed in E.coli, covering amino acids 1-462aa, with N-terminal 6xHis-tagged tag, molecular weight 57.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites (By similarity). Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells (By similarity). Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Represses the transcription of CYP2A5 (PubMed:30555544). Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (PubMed:29653076). Required for the development of natural killer cell precursors (PubMed:32190943)

Gene: Nfil3  |  Organism: Mus musculus

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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