Recombinant Mouse P2X purinoceptor 4 (P2rx4), partial Protein, Mus musculus (Mouse), in vitro E.coli expression system, N-terminal 6xHis-SUMO-tagged, 47.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse P2X purinoceptor 4 (P2rx4), partial Protein

Recombinant Mouse P2X purinoceptor 4 (P2rx4), partial Protein — ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium. Purity >90%.

SKU: BCRECP-00685TM Category:
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Product Specifications

Uniprot No.Q9JJX6
Gene NamesP2rx4
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region55-338aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight47.5kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium. CTP, but not GTP or UTP, functions as a weak affinity agonist for P2RX4. Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Mouse P2X purinoceptor 4 (P2rx4), partial Protein is a recombinant protein from Mus musculus (Mouse), expressed in in vitro E.coli expression system, covering amino acids 55-338aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 47.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and calcium. CTP, but not GTP or UTP, functions as a weak affinity agonist for P2RX4 (By similarity). Activated by extracellularly released ATP, it plays multiple role in immunity and central nervous system physiology (PubMed:26456657, PubMed:35119925). Plays a key role in initial steps of T-cell activation and Ca(2+) microdomain formation (PubMed:35119925). Also participates in basal T-cell activity without TCR/CD3 stimulation (PubMed:35119925). Promotes the differentiation and activation of Th17 cells via expression of retinoic acid-related orphan receptor C/RORC (By similarity). Upon activation, drives microglia motility via the PI3K/Akt pathway (PubMed:17299767). Could also function as an ATP-gated cation channel of lysosomal membranes (PubMed:27477609)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein)

Subunit

Functional P2RXs are organized as homomeric and heteromeric trimers. Functional P2XRs are organized as homomeric and heteromeric trimers. Forms heterotrimer with P2RX1 (By similarity). Interacts with P2RX7 (via C-terminus); this interaction is functional only in the presence of ATP (PubMed:26456657). Forms heterotrimer with P2RX4; functional differences between homomeric P2RX4 and P2RX4/6 heterotrimer are minor (By similarity). Interacts with AP1M2 (By similarity)

Gene: P2rx4  |  Organism: Mus musculus  |  Synonyms: ATP receptor; Purinergic receptor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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