Recombinant Mouse Perforin-1 (PRF1), Mus musculus (Mouse), Yeast, N-terminal 6xHis-tagged, 61.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Perforin-1 (PRF1)

Recombinant Mouse Perforin-1 (PRF1) — Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Purity >90%.

SKU: BCRECP-004157 Category:
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Product Specifications

Product SkuBCRECP-004157
Product DescriptionRecombinant Mouse Perforin-1 (PRF1) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 21-554aa. [Accession | P10820].
Uniprot No.P10820
Gene NamesPRF1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region21-554aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight61.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes.

Biological Context

Subcellular Location: Cytolytic granule; Secreted; Cell membrane (Multi-pass membrane protein); Endosome lumen
Tissue Specificity: Detected in cytotoxic T-lymphocytes and natural killer cells

Product Specifications

Recombinant Mouse Perforin-1 (PRF1) is a recombinant protein from Mus musculus (Mouse), expressed in Yeast, covering amino acids 21-554aa, with N-terminal 6xHis-tagged tag, molecular weight 61.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:19446473, PubMed:21037563, PubMed:26306037, PubMed:2783478, PubMed:3261391, PubMed:35148176, PubMed:35705808, PubMed:7520535, PubMed:7526382, PubMed:7972104, PubMed:8164737). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:19446473, PubMed:21037563, PubMed:26306037, PubMed:3261391, PubMed:35148176, PubMed:35705808, PubMed:7526382, PubMed:8164737). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:19446473, PubMed:21037563, PubMed:26306037, PubMed:3261391, PubMed:35148176, PubMed:7526382, PubMed:8164737). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (By similarity). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (PubMed:35705808)

Subcellular Location

Cytolytic granule; Secreted; Cell membrane (Multi-pass membrane protein); Endosome lumen

Tissue Specificity

Detected in cytotoxic T-lymphocytes and natural killer cells

Subunit

Monomer, as soluble protein (PubMed:19446473, PubMed:21037563). Homooligomer; homooligomerizes to form a pore-forming ring (PubMed:19446473, PubMed:21037563, PubMed:35148176)

Gene: Prf1  |  Organism: Mus musculus  |  Synonyms: Cytolysin; Lymphocyte pore-forming protein
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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