Recombinant Mouse Perforin-1 (PRF1), Mus musculus (Mouse), Yeast, N-terminal 6xHis-tagged, 61.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Perforin-1 (PRF1)

Recombinant Mouse Perforin-1 (PRF1) — Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Purity >90%.

SKU: BCRECP-004157 Category:
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Product Specifications

Product SkuBCRECP-004157
Product DescriptionRecombinant Mouse Perforin-1 (PRF1) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 21-554aa. [Accession | P10820].
Uniprot No.P10820
Gene NamesPRF1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region21-554aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight61.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes.

Biological Context

Subcellular Location: Cytolytic granule; Secreted; Cell membrane (Multi-pass membrane protein); Endosome lumen
Tissue Specificity: Detected in cytotoxic T-lymphocytes and natural killer cells

Product Specifications

Recombinant Mouse Perforin-1 (PRF1) is a recombinant protein from Mus musculus (Mouse), expressed in Yeast, covering amino acids 21-554aa, with N-terminal 6xHis-tagged tag, molecular weight 61.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:19446473, PubMed:21037563, PubMed:26306037, PubMed:2783478, PubMed:3261391, PubMed:35148176, PubMed:35705808, PubMed:7520535, PubMed:7526382, PubMed:7972104, PubMed:8164737). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:19446473, PubMed:21037563, PubMed:26306037, PubMed:3261391, PubMed:35148176, PubMed:35705808, PubMed:7526382, PubMed:8164737). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:19446473, PubMed:21037563, PubMed:26306037, PubMed:3261391, PubMed:35148176, PubMed:7526382, PubMed:8164737). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (By similarity). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (PubMed:35705808)

Subcellular Location

Cytolytic granule; Secreted; Cell membrane (Multi-pass membrane protein); Endosome lumen

Tissue Specificity

Detected in cytotoxic T-lymphocytes and natural killer cells

Subunit

Monomer, as soluble protein (PubMed:19446473, PubMed:21037563). Homooligomer; homooligomerizes to form a pore-forming ring (PubMed:19446473, PubMed:21037563, PubMed:35148176)

Gene: Prf1  |  Organism: Mus musculus  |  Synonyms: Cytolysin; Lymphocyte pore-forming protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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