Recombinant Mouse Peroxisomal coenzyme A diphosphatase NUDT7 (Nudt7), Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 33.9kDa, purity >85% as determined by SDS-PAGE.
,

Recombinant Mouse Peroxisomal coenzyme A diphosphatase NUDT7 (Nudt7)

Recombinant Mouse Peroxisomal coenzyme A diphosphatase NUDT7 (Nudt7) — Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4′-phosphopantetheine and adenosine 3′,5′-bisphosphate. Purity >85%.

SKU: BCRECP-003918 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-003918
Product DescriptionRecombinant Mouse Peroxisomal coenzyme A diphosphatase NUDT7 (Nudt7) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-236aa. [Accession | Q99P30].
Uniprot No.Q99P30
Gene NamesNudt7
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-236aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight33.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate. Cleaves CoA, CoA esters and oxidized CoA with similar efficiencies. Preferentially hydrolyzes medium-chain acyl-CoAs and bile acid-CoAs.

Biological Context

Subcellular Location: Peroxisome
Tissue Specificity: Expressed in liver, brown adipose tissue and heart at 20 times lower levels than isoform 1

Product Specifications

Recombinant Mouse Peroxisomal coenzyme A diphosphatase NUDT7 (Nudt7) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-236aa, with C-terminal 6xHis-tagged tag, molecular weight 33.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate (PubMed:11415433, PubMed:18799520, PubMed:29378847). Cleaves CoA, CoA esters and oxidized CoA with similar efficiencies (PubMed:11415433). Preferentially hydrolyzes medium-chain acyl-CoAs and bile acid-CoAs (PubMed:18799520). Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester (PubMed:18799520). May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand (PubMed:18799520). Does not play a role in U8 snoRNA decapping activity (PubMed:21070968). Binds U8 snoRNA (PubMed:21070968). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (PubMed:32432673)

Subcellular Location

Peroxisome

Tissue Specificity

Expressed in liver, brown adipose tissue and heart at 20 times lower levels than isoform 1

Subunit

Monomer

Gene: Nudt7  |  Organism: Mus musculus  |  Synonyms: Nucleoside diphosphate-linked moiety X motif 7
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top