Recombinant Mouse Platelet-derived growth factor subunit B (Pdgfb)

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA

Subcellular Location

Secreted

Tissue Specificity

Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4

Subunit

Antiparallel homodimer; disulfide-linked. Antiparallel heterodimer with PDGFA; disulfide-linked. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 (By similarity). Interacts with LRP1. Interacts with SORL1 (via the N-terminal ectodomain). Interacts with CD82; this interaction inhibits PDGFB-mediated signaling pathway (By similarity)

• Bonthron D.T. et al. Structure of the murine c-sis proto-oncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor. — Genomics (1991)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Leveen P. et al. Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities. — Genes Dev. (1994)
• Lindahl P. et al. Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. — Science (1997)
• Arar M. et al. Platelet-derived growth factor receptor beta regulates migration and DNA synthesis in metanephric mesenchymal cells. — J. Biol. Chem. (2000)
• Kaminski W.E. et al. Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF beta-receptor null mice. — Blood (2001)
• Taneda S. et al. Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury. — J. Am. Soc. Nephrol. (2003)
• Abramsson A. et al. Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors. — J. Clin. Invest. (2003)