Recombinant Mouse Protein c-Fos (Fos), Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 46.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Protein c-Fos (Fos)

Recombinant Mouse Protein c-Fos (Fos) — Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Purity >85%.

SKU: BCRECP-002778 Category:
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Product Specifications

Product SkuBCRECP-002778
Product DescriptionRecombinant Mouse Protein c-Fos (Fos) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-380aa. [Accession | P01101].
Uniprot No.P01101
Gene NamesFos
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-380aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight46.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex, at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton.

Biological Context

Subcellular Location: Nucleus; Endoplasmic reticulum; Cytoplasm, cytosol

Product Specifications

Recombinant Mouse Protein c-Fos (Fos) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-380aa, with N-terminal 6xHis-tagged tag, molecular weight 46.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex, at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling (By similarity). Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum

Subcellular Location

Nucleus; Endoplasmic reticulum; Cytoplasm, cytosol

Subunit

Heterodimer; with JUN (PubMed:29272704). Component of the SMAD3/SMAD4/JUN/FOS complex required for synergistic TGF-beta-mediated transcription at the AP1 promoter site (By similarity). Interacts with SMAD3; the interaction is weak even on TGF-beta activation (By similarity). Interacts with MAFB (By similarity). Interacts with TSC22D3 (via N-terminus); this interaction inhibits the binding of active AP1 to its target DNA (PubMed:11397794). Interacts with CDS1 and PI4K2A, but not with CDIPT, nor PI4K2B (PubMed:22105363). Interacts (via bZIP domain and leucine-zipper region) with the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF) subunits SMARCB1, SMARCC2 and SMARCD1 (PubMed:29272704). Interacts (via bZIP domain and leucine-zipper region) with ARID1A (PubMed:29272704)

Gene: Fos  |  Organism: Mus musculus  |  Synonyms: Cellular oncogene fos; FBJ osteosarcoma oncogene; Transcription factor AP-1 subunit c-Fos
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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