Recombinant Mouse Protein Wnt-7a (Wnt7a), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 43.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Protein Wnt-7a (Wnt7a)

Recombinant Mouse Protein Wnt-7a (Wnt7a) — Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Purity >90%.

SKU: BCRECP-004750 Category:
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Product Specifications

Product SkuBCRECP-004750
Product DescriptionRecombinant Mouse Protein Wnt-7a (Wnt7a) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 32-349aa. [Accession | P24383].
Uniprot No.P24383
Gene NamesWnt7a
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region32-349aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight43.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix; Secreted
Disease Association: [Defects in Wnt7a cause the postaxial hemimelia (px) phenotype that is characterized by limb patterning defects accompanied by Mullerian duct-associated sterility in both sexes]

Product Specifications

Recombinant Mouse Protein Wnt-7a (Wnt7a) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 32-349aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 43.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341, PubMed:20530549, PubMed:23629626). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:7885472, PubMed:9769174, PubMed:9790192). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:28803732). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (PubMed:9790192). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (PubMed:23629626). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (PubMed:23629626). Promotes formation of synapses via its interaction with FZD5 (PubMed:20530549)

Subcellular Location

Secreted, extracellular space, extracellular matrix; Secreted

Disease Association

[Defects in Wnt7a cause the postaxial hemimelia (px) phenotype that is characterized by limb patterning defects accompanied by Mullerian duct-associated sterility in both sexes]

Subunit

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (By similarity). The complex with AFM may represent the physiological form in body fluids (By similarity). Interacts with FZD5 (PubMed:18230341, PubMed:20530549). Interacts with PORCN (PubMed:10866835). Interacts (via intrinsically disordered linker region) with RECK; interaction with RECK confers ligand selectivity for Wnt7 in brain endothelial cells and allows these cells to selectively respond to Wnt7 (By similarity)

Gene: Wnt7a  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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