Recombinant Mouse Proto-oncogene Wnt-3 (Wnt3), Mus musculus (Mouse), E.coli, N-terminal 6xHis-SUMO-tagged, 53.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Proto-oncogene Wnt-3 (Wnt3)

Recombinant Mouse Proto-oncogene Wnt-3 (Wnt3) — Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Purity >90%.

SKU: BCRECP-004749 Category:
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Product Specifications

Product SkuBCRECP-004749
Product DescriptionRecombinant Mouse Proto-oncogene Wnt-3 (Wnt3) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 22-355aa. [Accession | P17553].
Uniprot No.P17553
Gene NamesWnt3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-355aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight53.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis.

Biological Context

Subcellular Location: Secreted, extracellular space, extracellular matrix; Secreted
Tissue Specificity: Detected at low levels in adult brain. Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds

Product Specifications

Recombinant Mouse Proto-oncogene Wnt-3 (Wnt3) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 22-355aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 53.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (By similarity). Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PubMed:10431240). Required for normal formation of the apical ectodermal ridge and for normal embryonic limb development (PubMed:12569130)

Subcellular Location

Secreted, extracellular space, extracellular matrix; Secreted

Tissue Specificity

Detected at low levels in adult brain (PubMed:2162045). Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds

Subunit

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids (By similarity). Interacts with PORCN (PubMed:10866835). Interacts with WLS (PubMed:19841259)

Gene: Wnt3  |  Organism: Mus musculus  |  Synonyms: Proto-oncogene Int-4

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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