Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active)

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (By similarity). Regulates GABAergic synapse development (PubMed:23699507, PubMed:29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (PubMed:23699507, PubMed:29981480). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (By similarity). Promotes the migration of cerebellar granule cells (PubMed:17554007). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (By similarity). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (By similarity)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues (PubMed:8969198). Expressed in neurons and glia in the developing hippocampus (PubMed:29981480)

Subunit

Homodimer (By similarity). Interacts with PLXNB1 (By similarity). Interacts with PLXNB2 (PubMed:17554007)

• Furuyama T. et al. Identification of a novel transmembrane semaphorin expressed on lymphocytes. — J. Biol. Chem. (1996)
• Mural R.J. et al. PubMed: CI-70VOHH7BF94TO (JUL-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Deng S. et al. Plexin-B2, but not Plexin-B1, critically modulates neuronal migration and patterning of the developing nervous system in vivo. — J. Neurosci. (2007)
• Wollscheid B. et al. Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins. — Nat. Biotechnol. (2009)
• Huttlin E.L. et al. A tissue-specific atlas of mouse protein phosphorylation and expression. — Cell (2010)
• Kuzirian M.S. et al. The class 4 semaphorin Sema4D promotes the rapid assembly of GABAergic synapses in rodent hippocampus. — J. Neurosci. (2013)
• McDermott J.E. et al. Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and glutamatergic synapse development in the mammalian hippocampus. — Mol. Cell. Neurosci. (2018)