Recombinant Mouse Sex-determining region Y protein (Sry), partial, Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 21.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Sex-determining region Y protein (Sry), partial

Recombinant Mouse Sex-determining region Y protein (Sry), partial — Constitutes a minor isoform, which is unstable due to the presence of a degron at the C-terminus that promotes its degradation. Purity >90%.

SKU: BCRECP-004484 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-004484
Product DescriptionRecombinant Mouse Sex-determining region Y protein (Sry) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-144aa. [Accession | Q05738].
Uniprot No.Q05738
Gene NamesSry
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-144aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight21.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Constitutes a minor isoform, which is unstable due to the presence of a degron at the C-terminus that promotes its degradation. Not necessary and sufficient for initiating male sex determination.

Biological Context

Subcellular Location: Nucleus speckle; Cytoplasm; Nucleus
Tissue Specificity: Expressed in gonadal somatic pre-Sertoli cells. While it is expressed at lower level compared to isoform Sry-S, this form is more stable and constitutes the predominant protein product of the Sry locus in XY gonads (at protein level)

Product Specifications

Recombinant Mouse Sex-determining region Y protein (Sry), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-144aa, with N-terminal 6xHis-tagged tag, molecular weight 21.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Constitutes a minor isoform, which is unstable due to the presence of a degron at the C-terminus that promotes its degradation (PubMed:33004521). Not necessary and sufficient for initiating male sex determination (PubMed:33004521)

Subcellular Location

Nucleus speckle; Cytoplasm; Nucleus

Tissue Specificity

Expressed in gonadal somatic pre-Sertoli cells (PubMed:33004521). While it is expressed at lower level compared to isoform Sry-S, this form is more stable and constitutes the predominant protein product of the Sry locus in XY gonads (at protein level) (PubMed:33004521)

Subunit

Interacts with KPNB1, ZNF208 isoform KRAB-O, PARP1 and SLC9A3R2 (PubMed:11535586, PubMed:15469996, PubMed:16166090, PubMed:16904257). The interaction with KPNB1 is sensitive to dissociation by Ran in the GTP-bound form (PubMed:11535586). Interaction with PARP1 impaired its DNA-binding activity (PubMed:16904257). Interacts with CALM, EP300, HDAC3 and WT1 (By similarity). The interaction with EP300 modulates its DNA-binding activity (By similarity)

Gene: Sry  |  Organism: Mus musculus  |  Synonyms: Testis-determining factor
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top