Recombinant Mouse Single-stranded DNA cytosine deaminase (Aicda), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 31.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Single-stranded DNA cytosine deaminase (Aicda)

Recombinant Mouse Single-stranded DNA cytosine deaminase (Aicda) — Single-stranded DNA-specific cytidine deaminase. Purity >90%.

SKU: BCRECP-002040 Category:
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Product Specifications

Product SkuBCRECP-002040
Product DescriptionRecombinant Mouse Single-stranded DNA cytosine deaminase (Aicda) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-198aa. [Accession | Q9WVE0].
Uniprot No.Q9WVE0
Gene NamesAicda
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-198aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight31.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses.

Biological Context

Subcellular Location: Nucleus; Cytoplasm
Tissue Specificity: Expressed in germinal center B-cells (at protein level)

Product Specifications

Recombinant Mouse Single-stranded DNA cytosine deaminase (Aicda) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-198aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 31.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation

Subcellular Location

Nucleus; Cytoplasm

Tissue Specificity

Expressed in germinal center B-cells (at protein level)

Subunit

Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 (By similarity). Interacts with SUPT6H, TRIM28 and NCL. Directly interacts with MCM3AP/GANP; this interaction may favor AICDA recruitment to immunoglobulin variable region genes, hence promoting somatic hypermutations (PubMed:20507984)

Gene: Aicda  |  Organism: Mus musculus  |  Synonyms: Activation-induced cytidine deaminase; Cytidine aminohydrolase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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