Recombinant Mouse Small proline-rich protein 2E (Sprr2e), Mus musculus (Mouse), E.coli, N-terminal 6xHis-SUMO-tagged, 21.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Small proline-rich protein 2E (Sprr2e)

Recombinant Mouse Small proline-rich protein 2E (Sprr2e) — Cross-linked envelope protein of keratinocytes. Purity >90%.

SKU: BCRECP-004476 Category:
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Product Specifications

Product SkuBCRECP-004476
Product DescriptionRecombinant Mouse Small proline-rich protein 2E (Sprr2e) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-76aa. [Accession | O70556].
Uniprot No.O70556
Gene NamesSprr2e
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-76aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight21.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Expressed in uterus

Product Specifications

Recombinant Mouse Small proline-rich protein 2E (Sprr2e) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-76aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 21.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane (By similarity)

Subcellular Location

Cytoplasm

Tissue Specificity

Expressed in uterus

Gene: Sprr2e  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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