Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active), Mus musculus (Mouse), E.coli, Tag-Free, 19.8 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active)

Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active) — The dually lipidated sonic hedgehog protein N-product (ShhN) is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during develop… Purity >95%.

SKU: BCACP-001111 Category:
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Product SKUBCACP-001111
Product DescriptionRecombinant Mouse Sonic hedgehog protein (Shh) protein is expressed from E.coli with Tag-Free. It contains 25-198aa. [Accession | Q62226].
Uniprot No.Q62226
Gene NamesShh
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-198aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight19.8 kDa
ActivityMeasured in a cell proliferation assay using NIH3T3 mouse embryonicfibroblast cells. The ED50 for this effect is ≤2.0 ng/mL
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesShh; Hhg1; Sonic hedgehog protein; SHH; HHG-1; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC) [Cleaved into: Sonic hedgehog protein N-product; ShhN; Shh N-terminal processed signaling domains; ShhNp; Sonic hedgehog protein 19 kDa product)]

Function

The dually lipidated sonic hedgehog protein N-product (ShhN) is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes. In the absence of SHH, patched represses the constitutive signaling activity of SMO.

Biological Context

Subcellular Location: Secreted; Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina

Product Specifications

Recombinant Mouse Sonic hedgehog protein (Shh), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 25-198aa, with Tag-Free tag, molecular weight 19.8 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The dually lipidated sonic hedgehog protein N-product (ShhN) is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development (PubMed:11389830, PubMed:14687547, PubMed:29622726, PubMed:7736596, PubMed:7916661). Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes (PubMed:31657721). In the absence of SHH, patched represses the constitutive signaling activity of SMO (PubMed:31657721). ShhN is widely expressed and plays a key role in the patterning of the central nervous system and limb development (PubMed:11430830, PubMed:12679031, PubMed:15315762, PubMed:24863049). Induces ventral cell fate in the neural tube and somites (PubMed:11430830, PubMed:24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (PubMed:15315762). Essential for axon guidance (PubMed:12679031)

Subcellular Location

Secreted; Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs (PubMed:7916661). In the adult, expressed in lung and neural retina (PubMed:7916661)

Subunit

Multimer (PubMed:15075292, PubMed:22677548, PubMed:22902404, PubMed:24522195). Interacts with SCUBE2; SCUBE2 acts as a carrier to promote release of the dual-lipidated sonic hedgehog protein N-product (ShhN) from the cell surface (PubMed:22677548, PubMed:24522195). Interacts with DISP1 via its cholesterol anchor (PubMed:22677548, PubMed:22902404). Interacts with BOC and CDON; BOC and CDON form a heterodimer that acts as a coreceptor for ShhN (PubMed:18794898, PubMed:20519495). Interacts with GAS1; GAS1 acts as a coreceptor for ShhN (PubMed:33038332)

Gene: Shh  |  Organism: Mus musculus  |  Synonyms: HHG-1; Shh unprocessed N-terminal signaling and C-terminal autoprocfretinaessing domains
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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