Recombinant Mouse Synaptosomal-associated protein 25 (Snap25), Mus musculus (Mouse), E.coli, Tag-Free, 23.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Synaptosomal-associated protein 25 (Snap25)

Recombinant Mouse Synaptosomal-associated protein 25 (Snap25) — t-SNARE involved in the molecular regulation of neurotransmitter release. Purity >90%.

SKU: BCRECP-004430 Category:
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Product Specifications

Product SkuBCRECP-004430
Product DescriptionRecombinant Mouse Synaptosomal-associated protein 25 (Snap25) Protein is expressed from E.coli with Tag-Free. It contains 1-206aa. [Accession | P60879].
Uniprot No.P60879
Gene NamesSnap25
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-206aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight23.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.

Biological Context

Subcellular Location: Cytoplasm, perinuclear region; Cell membrane (Lipid-anchor); Synapse, synaptosome; Photoreceptor inner segment
Tissue Specificity: Expressed in the outer nuclear layer of the retina (at protein level)

Product Specifications

Recombinant Mouse Synaptosomal-associated protein 25 (Snap25) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-206aa, with Tag-Free tag, molecular weight 23.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed:8103915, PubMed:8243676). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF (PubMed:16672379). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (By similarity)

Subcellular Location

Cytoplasm, perinuclear region; Cell membrane (Lipid-anchor); Synapse, synaptosome; Photoreceptor inner segment

Tissue Specificity

Expressed in the outer nuclear layer of the retina (at protein level)

Subunit

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex constitutes the basic catalytic machinery of the complex neurotransmitter release apparatus (PubMed:19196426, PubMed:28821673). Recruited to the SNARE complex following binding of the SNARE complex component STX1A to STXBP1 (PubMed:28821673). This complex binds CPLX1 (PubMed:19196426). Found in a complex containing SYT1, SV2B and STX1A (PubMed:15466855). Found in a ternary complex with STX1A and VAMP8 (PubMed:9731768). Interacts with HSC70 and with SYT9, forming a complex with DNAJC5 (PubMed:20847230). The interaction with SYT9 is inhibited in presence of calcium (PubMed:20847230). Isoform 1 and isoform 2 interact with BLOC1S6 (PubMed:19546860). Interacts with CENPF (PubMed:16672379). Interacts with EQTN (PubMed:19285662). Interacts with HGS (By similarity). Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells (By similarity). Interacts with OTOF (PubMed:17055430). Interacts with RIMS1 (By similarity). Interacts with SNAPIN (PubMed:10195194). Interacts with STXBP6 (By similarity). Interacts with TRIM9 (By similarity). Interacts with ZDHHC13 (via ANK repeats) (PubMed:25253725). Interacts with ZDHHC17 (via ANK repeats) (PubMed:25253725). Associates with the BLOC-1 complex (PubMed:19546860). Interacts with PLCL1 (via C2 domain) (PubMed:23341457). Interacts with PRRT2; this interaction may impair the formation of the SNARE complex (PubMed:22832103, PubMed:27052163, PubMed:29056747). Interacts with alpha-synuclein/SNCA (PubMed:20798282). Interacts with PRPH2 (PubMed:26406599). Interacts with ROM1 (PubMed:26406599). Interacts with STX3 isoform 3B (PubMed:26406599)

Gene: Snap25  |  Organism: Mus musculus  |  Synonyms: Super protein; Synaptosomal-associated 25 kDa protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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