Recombinant Mouse Tribbles homolog 3 (Trib3), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 46.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Tribbles homolog 3 (Trib3)

Recombinant Mouse Tribbles homolog 3 (Trib3) — Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Purity >85%.

SKU: BCRECP-004616 Category:
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Product Specifications

Product SkuBCRECP-004616
Product DescriptionRecombinant Mouse Tribbles homolog 3 (Trib3) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-354aa. [Accession | Q8K4K2].
Uniprot No.Q8K4K2
Gene NamesTrib3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-354aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight46.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Highly expressed in liver. Not detected in heart, brain, spleen, lung, skeletal muscle, kidney or testis

Product Specifications

Recombinant Mouse Tribbles homolog 3 (Trib3) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-354aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 46.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed:17369260). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (By similarity). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (By similarity). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (PubMed:12749859, PubMed:17369260). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (PubMed:12791994). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (PubMed:12791994). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (By similarity). Interacts with MAPK kinases and regulates activation of MAP kinases (By similarity). Can inhibit APOBEC3A editing of nuclear DNA (PubMed:22977230)

Subcellular Location

Nucleus

Tissue Specificity

Highly expressed in liver. Not detected in heart, brain, spleen, lung, skeletal muscle, kidney or testis

Subunit

Interacts with AKT1, AKT2, MAP2K1 and MAP2K7 (PubMed:12791994). Interacts with ATF4 (PubMed:12749859, PubMed:17369260). Interacts with DDIT3/CHOP and inhibits its interaction with EP300/P300 (By similarity). Interacts with APOBEC3C (By similarity). Interacts (via N-terminus) with APOBEC3A (PubMed:22977230). Interacts with RELA (By similarity)

Gene: Trib3  |  Organism: Mus musculus  |  Synonyms: Neuronal cell death-inducible putative kinase
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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