Recombinant Mouse Tripartite motif-containing protein 72 (Trim72), Mus musculus (Mouse), Yeast, N-terminal 6xHis-tagged, 53.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Tripartite motif-containing protein 72 (Trim72)

Recombinant Mouse Tripartite motif-containing protein 72 (Trim72) — Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Purity >85%.

SKU: BCRECP-004623 Category:
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Product Specifications

Product SkuBCRECP-004623
Product DescriptionRecombinant Mouse Tripartite motif-containing protein 72 (Trim72) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 1-477aa. [Accession | Q1XH17].
Uniprot No.Q1XH17
Gene NamesTrim72
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-477aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight53.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site.

Biological Context

Subcellular Location: Cell membrane, sarcolemma; Cytoplasmic vesicle membrane
Tissue Specificity: Muscle-specific. Exclusively expressed in cardiac and skeletal muscle
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Mouse Tripartite motif-containing protein 72 (Trim72) is a recombinant protein from Mus musculus (Mouse), expressed in Yeast, covering amino acids 1-477aa, with N-terminal 6xHis-tagged tag, molecular weight 53.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (PubMed:19043407, PubMed:37770719). Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3 (PubMed:37770719). Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (PubMed:19043407, PubMed:19202355). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (PubMed:19043407). Probably acts upstream of the Ca(2+)-dependent membrane resealing process (PubMed:19043407). Required for transport of DYSF to sites of cell injury during repair patch formation (PubMed:19043407). Regulates membrane budding and exocytosis (PubMed:19029292). May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (PubMed:19202355)

Subcellular Location

Cell membrane, sarcolemma; Cytoplasmic vesicle membrane

Tissue Specificity

Muscle-specific. Exclusively expressed in cardiac and skeletal muscle

Subunit

Homodimer (PubMed:37770719). Homooligomer; disulfide-linked (PubMed:19043407, PubMed:37770719). Oligomerizes on the phospholipid membrane (PubMed:37770719). Interacts with DYSF and CAV3 (PubMed:19380584)

Pathway

Protein modification; protein ubiquitination

Gene: Trim72  |  Organism: Mus musculus  |  Synonyms: Mitsugumin-53
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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