Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 60.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1)

Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1) — Involved in nitrogen metabolism via ammonium assimilation. Purity >90%.

SKU: BCRECP-002869 Category:
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Product Specifications

Product SkuBCRECP-002869
Product DescriptionRecombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-478aa. [Accession | P9WN39].
Uniprot No.P9WN39
Gene NamesglnA1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-478aa
SpeciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight60.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Also able to use GTP.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Mycobacterium tuberculosis Glutamine synthetase (glnA1) is a recombinant protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), expressed in E.coli, covering amino acids 2-478aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 60.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia (PubMed:12819079, PubMed:7937767). Also able to use GTP (PubMed:7937767). D-glutamate is a poor substrate, and DL-glutamate shows about 50% of the standard specific activity (PubMed:7937767). Also plays a key role in controlling the ammonia levels within infected host cells and so contributes to the pathogens capacity to inhibit phagosome acidification and phagosome-lysosome fusion (PubMed:12819079, PubMed:7937767). Involved in cell wall biosynthesis via the production of the major component poly-L-glutamine (PLG) (PubMed:10618433, PubMed:7937767). PLG synthesis in the cell wall occurs only in nitrogen limiting conditions and on the contrary high nitrogen conditions inhibit PLG synthesis (Probable)

Subcellular Location

Cytoplasm

Subunit

Oligomer of 12 subunits arranged in the form of two hexagons

Gene: glnA1  |  Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)  |  Synonyms: Glutamate--ammonia ligase; Glutamine synthetase I beta
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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