Recombinant Mycobacterium tuberculosis Large-conductance mechanosensitive channel (mscL), partial, Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 11.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis Large-conductance mechanosensitive channel (mscL), partial

Recombinant Mycobacterium tuberculosis Large-conductance mechanosensitive channel (mscL), partial — Channel that opens in response to stretch forces in the membrane lipid bilayer. Purity >90%.

SKU: BCRECP-003416 Category:
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Product Specifications

Product SkuBCRECP-003416
Product DescriptionRecombinant Mycobacterium tuberculosis Large-conductance mechanosensitive channel (mscL) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 91-151aa. [Accession | P9WJN5].
Uniprot No.P9WJN5
Gene NamesmscL
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region91-151aa
SpeciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight11.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Channel that opens in response to stretch forces in the membrane lipid bilayer. The force required to trigger channel opening depends on the nature of the membrane lipids; the presence of phosphatidylinositol enhances mechanosensitivity of the channel.

Product Specifications

Recombinant Mycobacterium tuberculosis Large-conductance mechanosensitive channel (mscL), partial — Channel that opens in response to stretch forces in the membrane lipid bilayer. Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Channel that opens in response to stretch forces in the membrane lipid bilayer. The force required to trigger channel opening depends on the nature of the membrane lipids; the presence of phosphatidylinositol enhances mechanosensitivity of the channel. May participate in the regulation of osmotic pressure changes within the cell

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Homopentamer

Gene: mscL  |  Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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