Recombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6), partial

Recombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6), partial — Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium and plays a major role in gliding motility. Purity >85%.

SKU: BCRECP-003973 Category:

Product Specifications

Product SkuBCRECP-003973
Product DescriptionRecombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 487-718aa. [Accession | Q50341].
Uniprot No.Q50341
Gene Namesorf6
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region487-718aa
SpeciesMycoplasma pneumoniae (strain ATCC 29342 / M129)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight32.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium and plays a major role in gliding motility. Binds sialylated oligosaccharides, which are present on the surface of its human host cells and which probably serve as the main host cell receptors for this bacterium. This protein and P1 mutually stabilize each other, and are required for formation of the attachment organelle.

Biological Context

Subcellular Location: Cell projection, attachment organelle membrane (Single-pass membrane protein); Cell surface

Product Specifications

Recombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6), partial is a recombinant protein from Mycoplasma pneumoniae (strain ATCC 29342 / M129), expressed in E.coli, covering amino acids 487-718aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 32.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium (PubMed:1904124, PubMed:33057023) and plays a major role in gliding motility (PubMed:40153444). Binds sialylated oligosaccharides, which are present on the surface of its human host cells and which probably serve as the main host cell receptors for this bacterium (PubMed:33057023, PubMed:39226978). This protein and P1 mutually stabilize each other, and are required for formation of the attachment organelle (By similarity)

Subcellular Location

Cell projection, attachment organelle membrane (Single-pass membrane protein); Cell surface

Subunit

Forms a heterodimer with P1 (PubMed:33057023). After proteolysis P40 and P90 remain associated; under denaturing conditions P40 can be lost from the complex (PubMed:33057023). The Nap adhesion complex consists of 2 P1-P40/P90 heterodimers (By similarity)

Gene: orf6  |  Organism: Mycoplasmoides pneumoniae (strain ATCC 29342 / M129 / Subtype 1)
Key Publications

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How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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