Recombinant Naja kaouthia Cobra venom factor, partial, Naja kaouthia (Monocled cobra) (Naja siamensis), E.coli, N-terminal GST-tagged, 55.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Naja kaouthia Cobra venom factor, partial

Recombinant Naja kaouthia Cobra venom factor, partial — Complement-activating protein in cobra venom. Purity >90%.

SKU: BCRECP-003760 Category:
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Product Specifications

Product SkuBCRECP-003760
Product DescriptionRecombinant Naja kaouthia Cobra venom factor, partial Protein is expressed from E.coli with N-terminal GST-tagged. It contains 733-984aa. [Accession | Q91132].
Uniprot No.Q91132
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region733-984aa
SpeciesNaja kaouthia (Monocled cobra) (Naja siamensis)
Tag InfoN-terminal GST-tagged
Molecular weight55.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Complement-activating protein in cobra venom. It is a structural and functional analog of complement component C3b, the activated form of C3. It binds factor B (CFB), which is subsequently cleaved by factor D (CFD) to form the bimolecular complex CVF/Bb.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the venom gland

Product Specifications

Recombinant Naja kaouthia Cobra venom factor, partial is a recombinant protein from Naja kaouthia (Monocled cobra) (Naja siamensis), expressed in E.coli, covering amino acids 733-984aa, with N-terminal GST-tagged tag, molecular weight 55.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Complement-activating protein in cobra venom. It is a structural and functional analog of complement component C3b, the activated form of C3. It binds factor B (CFB), which is subsequently cleaved by factor D (CFD) to form the bimolecular complex CVF/Bb. CVF/Bb is a C3/C5 convertase that cleaves both complement components C3 and C5. Structurally, it resembles the C3b degradation product C3c, which is not able to form a C3/C5 convertase. Unlike C3b/Bb, CVF/Bb is a stable complex and completely resistant to the actions of complement regulatory factors H (CFH) and I (CFI). Therefore, CVF continuously activates complement resulting in the depletion of complement activity

Subcellular Location

Secreted

Tissue Specificity

Expressed by the venom gland

Subunit

Heterotrimer of alpha, beta and gamma chains; disulfide-linked. Is active with factor B in the presence of factor D

Organism: Naja kaouthia  |  Synonyms: Complement C3 homolog
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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