Recombinant Neisseria meningitidis serogroup B Neisseria adhesin A (nadA), partial, Neisseria meningitidis serogroup B (strain MC58), E.coli, N-terminal 10xHis-tagged, 35.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Neisseria meningitidis serogroup B Neisseria adhesin A (nadA), partial

Recombinant Neisseria meningitidis serogroup B Neisseria adhesin A (nadA), partial — Adheres to and induces bacterial uptake by human epithelial cells (Probable). Purity >85%.

SKU: BCRECP-003839 Category:
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Product Specifications

Product SkuBCRECP-003839
Product DescriptionRecombinant Neisseria meningitidis serogroup B Neisseria adhesin A (nadA) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 24-306aa. [Accession | Q9JXK7].
Uniprot No.Q9JXK7
Gene NamesnadA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-306aa
SpeciesNeisseria meningitidis serogroup B (strain MC58)
Tag InfoN-terminal 10xHis-tagged
Molecular weight35.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Adheres to and induces bacterial uptake by human epithelial cells (Probable). Upon expression in engineered Y.enterocolitica confers an 11- to 15-fold increase in bacterial adherence and uptake by human epithelial cell lines; part of the uptake is mediated by integrin beta-1 (ITGB1) suggesting it may be a human receptor for NadA. A bacterial cell surface protein; antisera against this protein induce complement-mediated killing of this and other strains.

Biological Context

Subcellular Location: Cell surface; Cell outer membrane

Product Specifications

Recombinant Neisseria meningitidis serogroup B Neisseria adhesin A (nadA), partial is a recombinant protein from Neisseria meningitidis serogroup B (strain MC58), expressed in E.coli, covering amino acids 24-306aa, with N-terminal 10xHis-tagged tag, molecular weight 35.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Adheres to and induces bacterial uptake by human epithelial cells (Probable). Upon expression in engineered Y.enterocolitica confers an 11- to 15-fold increase in bacterial adherence and uptake by human epithelial cell lines; part of the uptake is mediated by integrin beta-1 (ITGB1) suggesting it may be a human receptor for NadA (PubMed:21471204). A bacterial cell surface protein; antisera against this protein induce complement-mediated killing of this and other strains (PubMed:12045242)

Subcellular Location

Cell surface; Cell outer membrane

Subunit

Forms high molecular weight oligomers in whole cell extracts that are not disrupted by boiling in SDS buffer (PubMed:12045242). Homotrimer (Probable). A fragment containing the N-terminal half of the mature protein (residues 24-210, head domain plus part of the stalk) binds human integrin beta-1 (ITGB1). It was not seen to bind immobilized purified CEACAMs 1, 3, 5, 6 or 8 nor commercially prepared type I collagen, fibronectin or matrigel (PubMed:21471204)

Gene: nadA  |  Organism: Neisseria meningitidis serogroup B (strain ATCC BAA-335 / MC58)
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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