Recombinant Neosartorya fumigata Probable glycosidase crf1 (crf1), partial, Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Neosartorya fumigata Probable glycosidase crf1 (crf1), partial

Recombinant Neosartorya fumigata Probable glycosidase crf1 (crf1), partial — Dual chitinase/transglycosylase that plays a role in cell wall architecture. Purity >85%.

SKU: BCRECP-002433 Category:
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Product Specifications

Product SkuBCRECP-002433
Product DescriptionRecombinant Neosartorya fumigata Probable glycosidase crf1 (crf1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 42-233aa. [Accession | Q8J0P4].
Uniprot No.Q8J0P4
Gene Namescrf1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region42-233aa
SpeciesNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMetabolism

Function

Dual chitinase/transglycosylase that plays a role in cell wall architecture. Chitinase and transglycosylase activities are coupled. Required for the polysaccharide cross-linking at the septa and the cell wall.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor); Secreted, cell wall

Product Specifications

Recombinant Neosartorya fumigata Probable glycosidase crf1 (crf1), partial is a recombinant protein from Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus), expressed in E.coli, covering amino acids 42-233aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Dual chitinase/transglycosylase that plays a role in cell wall architecture (PubMed:30971696). Chitinase and transglycosylase activities are coupled (PubMed:30971696). Required for the polysaccharide cross-linking at the septa and the cell wall (PubMed:30971696). More specifically, transfers chitin to 1,6-beta-glucan in the cell wall (PubMed:30971696). Chr5 shows acceptor substrate promiscuity and is also able to cross-link chitin to chitin (PubMed:30971696)

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor); Secreted, cell wall

Gene: crh5  |  Organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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