Recombinant Newcastle disease virus Nucleoprotein (N), Newcastle disease virus (strain Chicken/United States/B1/48) (NDV), E.coli, N-terminal 6xHis-tagged, 57.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Newcastle disease virus Nucleoprotein (N)

Recombinant Newcastle disease virus Nucleoprotein (N) — Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. Purity >90%.

SKU: BCRECP-003493 Categories: ,
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Product Specifications

Product SkuBCRECP-003493
Product DescriptionRecombinant Newcastle disease virus Nucleoprotein (N) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-489aa. [Accession | Q77K03].
Uniprot No.Q77K03
Gene NamesN
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-489aa
SpeciesNewcastle disease virus (strain Chicken/United States/B1/48) (NDV)
Tag InfoN-terminal 6xHis-tagged
Molecular weight57.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis.

Product Specifications

Recombinant Newcastle disease virus Nucleoprotein (N) — Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. Purity >90%. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases (By similarity). The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P (By similarity)

Subcellular Location

Virion; Host cytoplasm

Subunit

Homomultimer; forms the nucleocapsid (By similarity). Binds to the viral genomic RNA (By similarity). N0 interacts with the phosphoprotein (via N-terminus); this interaction allows P to chaperon N0 to avoid N polymerization before encapsidation. Interacts as N-RNA template with the phosphoprotein (via C-terminus); this interaction positions the polymerase on the template (By similarity)

Gene: N  |  Organism: Newcastle disease virus (strain Chicken/United States/B1/48)  |  Synonyms: Nucleocapsid protein

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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