Recombinant Oryza sativa subsp. japonica Ribulose bisphosphate carboxylase large chain (rbcL)

Recombinant Oryza sativa subsp. japonica Ribulose bisphosphate carboxylase large chain (rbcL) — RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in t…

SKU: BCRECP-004237 Category:

Product Specifications

Product SkuBCRECP-004237
Product DescriptionRecombinant Oryza sativa subsp. japonica Ribulose bisphosphate carboxylase large chain (rbcL) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 3-477aa. [Accession | P0C512].
Uniprot No.P0C512
Gene NamesrbcL
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region3-477aa
SpeciesOryza sativa subsp. japonica (Rice)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight60.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.

Biological Context

Subcellular Location: Plastid, chloroplast

Product Specifications

Recombinant Oryza sativa subsp. japonica Ribulose bisphosphate carboxylase large chain (rbcL) is a recombinant protein from Oryza sativa subsp. japonica (Rice), expressed in E.coli, covering amino acids 3-477aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 60.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site

Subcellular Location

Plastid, chloroplast

Subunit

Heterohexadecamer of 8 large chains and 8 small chains (PubMed:22609438); disulfide-linked. The disulfide link is formed within the large subunit homodimers

Gene: rbcL  |  Organism: Oryza sativa subsp. japonica

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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