Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5), Sus scrofa (Pig), Yeast, N-terminal 6xHis-tagged and C-terminal Myc-tagged, 26.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5)

Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5) — May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Purity >90%.

SKU: BCRECP-002908 Categories: ,
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Product Specifications

Product SkuBCRECP-002908
Product DescriptionRecombinant Pig Epididymal secretory glutathione peroxidase (GPX5) Protein is expressed from Yeast with N-terminal 6xHis-tagged and C-terminal Myc-tagged. It contains 22-219aa. [Accession | O18994].
Uniprot No.O18994
Gene NamesGPX5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region22-219aa
SpeciesSus scrofa (Pig)
Tag InfoN-terminal 6xHis-tagged and C-terminal Myc-tagged
Molecular weight26.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Proximal caput epididymis

Product Specifications

Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5) is a recombinant protein from Sus scrofa (Pig), expressed in Yeast, covering amino acids 22-219aa, with N-terminal 6xHis-tagged and C-terminal Myc-tagged tag, molecular weight 26.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction

Subcellular Location

Secreted

Tissue Specificity

Proximal caput epididymis

Subunit

Homotetramer

Gene: GPX5  |  Organism: Sus scrofa  |  Synonyms: Epididymis-specific glutathione peroxidase-like protein; Glutathione peroxidase 5

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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