Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5), Sus scrofa (Pig), Yeast, N-terminal 6xHis-tagged and C-terminal Myc-tagged, 26.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5)

Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5) — May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Purity >90%.

SKU: BCRECP-002908 Categories: ,
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Product Specifications

Product SkuBCRECP-002908
Product DescriptionRecombinant Pig Epididymal secretory glutathione peroxidase (GPX5) Protein is expressed from Yeast with N-terminal 6xHis-tagged and C-terminal Myc-tagged. It contains 22-219aa. [Accession | O18994].
Uniprot No.O18994
Gene NamesGPX5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region22-219aa
SpeciesSus scrofa (Pig)
Tag InfoN-terminal 6xHis-tagged and C-terminal Myc-tagged
Molecular weight26.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Proximal caput epididymis

Product Specifications

Recombinant Pig Epididymal secretory glutathione peroxidase (GPX5) is a recombinant protein from Sus scrofa (Pig), expressed in Yeast, covering amino acids 22-219aa, with N-terminal 6xHis-tagged and C-terminal Myc-tagged tag, molecular weight 26.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction

Subcellular Location

Secreted

Tissue Specificity

Proximal caput epididymis

Subunit

Homotetramer

Gene: GPX5  |  Organism: Sus scrofa  |  Synonyms: Epididymis-specific glutathione peroxidase-like protein; Glutathione peroxidase 5

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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