Recombinant Pig Vasopressin V2 receptor (AVPR2), partial, Sus scrofa (Pig), E.coli, N-terminal 6xHis-SUMO-tagged, 24.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Pig Vasopressin V2 receptor (AVPR2), partial

Recombinant Pig Vasopressin V2 receptor (AVPR2), partial — G protein-coupled receptor for arginine vasopressin, an antidiuretic that promotes renal water reabsorption. Purity >90%.

SKU: BCRECP-002168 Category:
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Product Specifications

Product SkuBCRECP-002168
Product DescriptionRecombinant Pig Vasopressin V2 receptor (AVPR2) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 292-370aa. [Accession | P32307].
Uniprot No.P32307
Gene NamesAVPR2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region292-370aa
SpeciesSus scrofa (Pig)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight24.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

G protein-coupled receptor for arginine vasopressin, an antidiuretic that promotes renal water reabsorption. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). AVPR2 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Pig Vasopressin V2 receptor (AVPR2), partial is a recombinant protein from Sus scrofa (Pig), expressed in E.coli, covering amino acids 292-370aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 24.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled receptor for arginine vasopressin, an antidiuretic that promotes renal water reabsorption (PubMed:8393786). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP) (By similarity). AVPR2 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Interacts with ARRDC4. Identified in a complex containing at least ARRDC4, V2R and HGS. Interacts with TMEM147. Interacts (when phosphorylated) with ARRB2; the interaction is associated with internalization of the receptor and short-term desensitization to the ligand

Gene: AVPR2  |  Organism: Sus scrofa  |  Synonyms: AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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