Recombinant Pisum sativum Leghemoglobin Lb120-8, Pisum sativum (Garden pea), E.coli, N-terminal 6xHis-tagged, 19.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Pisum sativum Leghemoglobin Lb120-8

Recombinant Pisum sativum Leghemoglobin Lb120-8 — Leghemoglobin that reversibly binds oxygen O(2) through a pentacoordinated heme iron. Purity >90%.

SKU: BCRECP-003766 Category:
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Product Specifications

Product SkuBCRECP-003766
Product DescriptionRecombinant Pisum sativum Leghemoglobin Lb120-8 Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-146aa. [Accession | Q9SAZ1].
Uniprot No.Q9SAZ1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-146aa
SpeciesPisum sativum (Garden pea)
Tag InfoN-terminal 6xHis-tagged
Molecular weight19.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Leghemoglobin that reversibly binds oxygen O(2) through a pentacoordinated heme iron. In root nodules, facilitates the diffusion of oxygen to the bacteroids while preventing the bacterial nitrogenase from being inactivated by buffering dioxygen, nitric oxide and carbon monoxide, and promoting the formation of reactive oxygen species (ROS, e.g. H(2)O(2)).

Biological Context

Subcellular Location: Cytoplasm, cytosol; Nucleus
Tissue Specificity: Root nodules

Product Specifications

Recombinant Pisum sativum Leghemoglobin Lb120-8 is a recombinant protein from Pisum sativum (Garden pea), expressed in E.coli, covering amino acids 2-146aa, with N-terminal 6xHis-tagged tag, molecular weight 19.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Leghemoglobin that reversibly binds oxygen O(2) through a pentacoordinated heme iron (By similarity). In root nodules, facilitates the diffusion of oxygen to the bacteroids while preventing the bacterial nitrogenase from being inactivated by buffering dioxygen, nitric oxide and carbon monoxide, and promoting the formation of reactive oxygen species (ROS, e.g. H(2)O(2)) (By similarity). This role is essential for symbiotic nitrogen fixation (SNF) (By similarity)

Subcellular Location

Cytoplasm, cytosol; Nucleus

Tissue Specificity

Root nodules

Subunit

Monomer

Organism: Pisum sativum

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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