Recombinant Plasmodium falciparum Plasmepsin I (PMI), Plasmodium falciparum (isolate 3D7), E.coli, N-terminal 6xHis-SUMO-tagged, 52.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Plasmodium falciparum Plasmepsin I (PMI)

Recombinant Plasmodium falciparum Plasmepsin I (PMI) — During the asexual blood stage, catalyzes the initial cleavage of native host hemoglobin (Hb) resulting in Hb denaturation; specifically cleaves between Phe-33 and Leu-34 of Hb alpha-chain. Purity >90%.

SKU: BCRECP-004120 Category:
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Product Specifications

Product SkuBCRECP-004120
Product DescriptionRecombinant Plasmodium falciparum Plasmepsin I (PMI) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 125-452aa. [Accession | Q7KQM4].
Uniprot No.Q7KQM4
Gene NamesPMI
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region125-452aa
SpeciesPlasmodium falciparum (isolate 3D7)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight52.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

During the asexual blood stage, catalyzes the initial cleavage of native host hemoglobin (Hb) resulting in Hb denaturation; specifically cleaves between Phe-33 and Leu-34 of Hb alpha-chain. Digestion of host Hb is an essential step which provides the parasite with amino acids for protein synthesis, and regulates osmolarity.

Biological Context

Subcellular Location: Membrane (Single-pass type II membrane protein); Vacuole lumen; Vacuole membrane

Product Specifications

Recombinant Plasmodium falciparum Plasmepsin I (PMI) is a recombinant protein from Plasmodium falciparum (isolate 3D7), expressed in E.coli, covering amino acids 125-452aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 52.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

During the asexual blood stage, catalyzes the initial cleavage of native host hemoglobin (Hb) resulting in Hb denaturation; specifically cleaves between Phe-33 and Leu-34 of Hb alpha-chain. Digestion of host Hb is an essential step which provides the parasite with amino acids for protein synthesis, and regulates osmolarity

Subcellular Location

Membrane (Single-pass type II membrane protein); Vacuole lumen; Vacuole membrane

Gene: PMI  |  Organism: Plasmodium falciparum (isolate 3D7)  |  Synonyms: Aspartic hemoglobinase I; Plasmepsin 1

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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