Recombinant Poecilobdella viridis Hirudin (Active), Poecilobdella viridis (Indian freshwater leech), Yeast, Tag-Free, 6.7 kDa, purity >>96% as determined by SDS-PAGE and HPLC.

Recombinant Poecilobdella viridis Hirudin (Active)

Recombinant Poecilobdella viridis Hirudin (Active) — Hirudin is a potent thrombin-specific protease inhibitor. Purity >96%.

SKU: BCACP-000859 Category:
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Product SKUBCACP-000859
Product DescriptionRecombinant Indian freshwater leech Hirudin protein is expressed from Yeast with Tag-Free. It contains 1-63aa. [Accession | P84590].
Uniprot No.P84590
Gene NamesN/A
Purity>96% as determined by SDS-PAGE and HPLC.
Expression SystemYeast
Expression Region1-63aa
SpeciesPoecilobdella viridis (Indian freshwater leech)
Tag InfoTag-Free
Molecular weight6.7 kDa
ActivityFully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of >100 IU/mg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesHirudin

Function

Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Poecilobdella viridis Hirudin (Active) is a recombinant protein from Poecilobdella viridis (Indian freshwater leech), expressed in Yeast, covering amino acids 1-63aa, with Tag-Free tag, molecular weight 6.7 kDa, purity >96% as determined by SDS-PAGE and HPLC.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of >100 IU/mg.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen

Subcellular Location

Secreted

Organism: Poecilobdella viridis

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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