Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla), Pseudomonas aeruginosa, E.coli, N-terminal 6xHis-SUMO-tagged, 43.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla)

Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla) — Class D beta-lactamase which confers resistance to the beta-lactam antibiotics, including penicillin, carbenicillin and oxacillin, and also some cephalosporins. Purity >90%.

SKU: BCRECP-002203 Category:
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Product Specifications

Product SkuBCRECP-002203
Product DescriptionRecombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 21-266aa. [Accession | P14489].
Uniprot No.P14489
Gene Namesbla
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-266aa
SpeciesPseudomonas aeruginosa
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight43.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Class D beta-lactamase which confers resistance to the beta-lactam antibiotics, including penicillin, carbenicillin and oxacillin, and also some cephalosporins. Confers weak resistance to some carbapenems, in E.coli strain C600Z1. Acts via hydrolysis of the beta-lactam ring.

Biological Context

Subcellular Location: Periplasm

Product Specifications

Recombinant Pseudomonas aeruginosa Beta-lactamase OXA-10 (bla) is a recombinant protein from Pseudomonas aeruginosa, expressed in E.coli, covering amino acids 21-266aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 43.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Class D beta-lactamase which confers resistance to the beta-lactam antibiotics, including penicillin, carbenicillin and oxacillin, and also some cephalosporins (PubMed:11724923, PubMed:36129295, PubMed:8215276). Confers weak resistance to some carbapenems, in E.coli strain C600Z1 (PubMed:30397053). Acts via hydrolysis of the beta-lactam ring (PubMed:11724923, PubMed:19860471, PubMed:30397053, PubMed:8215276). Has penicillin- and cephalosporin-hydrolyzing activities (PubMed:11724923, PubMed:19860471, PubMed:30397053)

Subcellular Location

Periplasm

Subunit

Dimer

Gene: OXA-10  |  Organism: Pseudomonas aeruginosa  |  Synonyms: Beta-lactamase PSE-2
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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