Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF), Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 16.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF)

Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF) — Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells, facilitating the establishment and dissem… Purity >85%.

SKU: BCRECP-004188 Category:
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Product Specifications

Product SkuBCRECP-004188
Product DescriptionRecombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-85aa. [Accession | P95434].
Uniprot No.P95434
Gene NamespscF
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-85aa
SpeciesPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight16.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells, facilitating the establishment and dissemination of infection. PscF/SctF forms the external needle filament that protrudes from the bacterial surface.

Biological Context

Subcellular Location: Secreted; Cell surface

Product Specifications

Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF) is a recombinant protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1), expressed in E.coli, covering amino acids 2-85aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 16.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells, facilitating the establishment and dissemination of infection. PscF/SctF forms the external needle filament that protrudes from the bacterial surface

Subcellular Location

Secreted; Cell surface

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (By similarity). This subunit polymerizes and forms the helical needle filament (PubMed:16115870). Forms a stable heterotrimeric complex with PscE and PscG in the cytoplasm, blocking it in a monomeric state and preventing its polymerization (PubMed:16115870, PubMed:17470796)

Gene: sctF  |  Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)  |  Synonyms: Pseudomonas secretion protein F; Type III needle protein PscF
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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