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Recombinant Pseudomonas sp. Cyanuric acid amidohydrolase (atzD)

Recombinant Pseudomonas sp. Cyanuric acid amidohydrolase (atzD) — Responsible for the hydrolysis of cyanuric acid, an intermediate formed during catabolism of s-triazine based compounds in herbicides such as atrazine and polymers such as melamine. Purity >90%.

SKU: BCRECP-002166 Categories: ,

Product Specifications

Product SkuBCRECP-002166
Product DescriptionRecombinant Pseudomonas sp. Cyanuric acid amidohydrolase (atzD) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-363aa. [Accession | P58329].
Uniprot No.P58329
Gene NamesatzD
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-363aa
SpeciesPseudomonas sp. (strain ADP)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight45.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Responsible for the hydrolysis of cyanuric acid, an intermediate formed during catabolism of s-triazine based compounds in herbicides such as atrazine and polymers such as melamine. Catalyzes the hydrolytic opening of the s-triazine ring of cyanuric acid (2,4,6-trihydroxy-s-triazine) to yield carbon dioxide and carboxybiuret, which spontaneously decarboxylates to biuret.

Biological Context

Pathway: Xenobiotic degradation; atrazine degradation; biuret from cyanurate: step 1/1

Product Specifications

Recombinant Pseudomonas sp. Cyanuric acid amidohydrolase (atzD) is a recombinant protein from Pseudomonas sp. (strain ADP), expressed in E.coli, covering amino acids 1-363aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 45.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Responsible for the hydrolysis of cyanuric acid, an intermediate formed during catabolism of s-triazine based compounds in herbicides such as atrazine and polymers such as melamine. Catalyzes the hydrolytic opening of the s-triazine ring of cyanuric acid (2,4,6-trihydroxy-s-triazine) to yield carbon dioxide and carboxybiuret, which spontaneously decarboxylates to biuret

Subunit

Homotetramer

Pathway

Xenobiotic degradation; atrazine degradation; biuret from cyanurate: step 1/1

Gene: atzD  |  Organism: Pseudomonas sp. (strain ADP)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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