Recombinant Puumala virus Nucleoprotein (N) — Encapsidates the genome protecting it from nucleases (Probable). Purity >95%.
| Product Sku | BCRECP-003459 |
| Product Description | Recombinant Puumala virus Nucleoprotein (N) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-433aa. [Accession | P19475]. |
| Uniprot No. | P19475 |
| Gene Names | N |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-433aa |
| Species | Puumala virus (strain Bank vole/Russia/CG1820/1984) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 56.5kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable).
Recombinant Puumala virus Nucleoprotein (N) — Encapsidates the genome protecting it from nucleases (Probable). Purity >95%. Suitable for ELISA and Western Blot applications. Explore more Viral Antigen proteins →
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable). The nucleocapsid has a left-handed helical structure (By similarity). As a trimer, specifically binds and acts as a chaperone to unwind the panhandle structure formed by the viral RNA (vRNA) termini (By similarity). Involved in the transcription and replication initiation of vRNA by mediating primer annealing (By similarity). Plays a role in cap snatching by sequestering capped RNAs in P bodies for use by the viral RdRp during transcription initiation (By similarity). Substitutes for the cellular cap-binding complex (eIF4F) to preferentially facilitate the translation of capped mRNAs (By similarity). Initiates the translation by specifically binding to the cap and 40S ribosomal subunit (By similarity). Prevents the viral glycoprotein N (Gn) from autophagy-dependent breakdown maybe by blocking autophagosome formation (By similarity). Inhibits host EIF2AK2/PKR dimerization to prevent PKR-induced translational shutdown in cells and thus the activation of the antiviral state (By similarity). Also displays sequence-unspecific DNA endonuclease activity (By similarity)
Virion; Host cytoplasm, host perinuclear region; Host Golgi apparatus, host cis-Golgi network
Homotrimer (By similarity). Homomultimer (By similarity). Homomultimerizes and binds to viral genomic RNA to form the nucleocapsid (By similarity). Interacts with host MAP1LC3B; this interaction participates to the protection of Gn from virus-triggered autophagy (By similarity). Interacts with host SNAP29; this interaction participates to the protection of glycoprotein N from virus-triggered autophagy (By similarity). Interacts (via N-terminus) with host RPS19; this interaction probably mediates the loading of the 40S ribosomal subunit on viral capped mRNA during N-mediated translation initiation (By similarity). Interacts with the viral RdRp (By similarity). Interacts with host SUMO1 (via N-terminus) (By similarity). Interacts with host DAXX (By similarity). Interacts with the viral glycoprotein N (via C-terminus) (By similarity). Interacts with the viral glycoprotein C (via C-terminus) (By similarity)
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Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
