Recombinant Puumala virus Nucleoprotein (N) — Encapsidates the genome protecting it from nucleases (Probable). Purity >95%.
| Product Sku | BCRECP-003459 |
| Product Description | Recombinant Puumala virus Nucleoprotein (N) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-433aa. [Accession | P19475]. |
| Uniprot No. | P19475 |
| Gene Names | N |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 1-433aa |
| Species | Puumala virus (strain Bank vole/Russia/CG1820/1984) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 56.5kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable).
Recombinant Puumala virus Nucleoprotein (N) — Encapsidates the genome protecting it from nucleases (Probable). Purity >95%. Suitable for ELISA and Western Blot applications. Explore more Viral Antigen proteins →
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Please contact us to obtain bioactivity data.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable). The nucleocapsid has a left-handed helical structure (By similarity). As a trimer, specifically binds and acts as a chaperone to unwind the panhandle structure formed by the viral RNA (vRNA) termini (By similarity). Involved in the transcription and replication initiation of vRNA by mediating primer annealing (By similarity). Plays a role in cap snatching by sequestering capped RNAs in P bodies for use by the viral RdRp during transcription initiation (By similarity). Substitutes for the cellular cap-binding complex (eIF4F) to preferentially facilitate the translation of capped mRNAs (By similarity). Initiates the translation by specifically binding to the cap and 40S ribosomal subunit (By similarity). Prevents the viral glycoprotein N (Gn) from autophagy-dependent breakdown maybe by blocking autophagosome formation (By similarity). Inhibits host EIF2AK2/PKR dimerization to prevent PKR-induced translational shutdown in cells and thus the activation of the antiviral state (By similarity). Also displays sequence-unspecific DNA endonuclease activity (By similarity)
Virion; Host cytoplasm, host perinuclear region; Host Golgi apparatus, host cis-Golgi network
Homotrimer (By similarity). Homomultimer (By similarity). Homomultimerizes and binds to viral genomic RNA to form the nucleocapsid (By similarity). Interacts with host MAP1LC3B; this interaction participates to the protection of Gn from virus-triggered autophagy (By similarity). Interacts with host SNAP29; this interaction participates to the protection of glycoprotein N from virus-triggered autophagy (By similarity). Interacts (via N-terminus) with host RPS19; this interaction probably mediates the loading of the 40S ribosomal subunit on viral capped mRNA during N-mediated translation initiation (By similarity). Interacts with the viral RdRp (By similarity). Interacts with host SUMO1 (via N-terminus) (By similarity). Interacts with host DAXX (By similarity). Interacts with the viral glycoprotein N (via C-terminus) (By similarity). Interacts with the viral glycoprotein C (via C-terminus) (By similarity)
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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
