Recombinant Rabbit Methylosome subunit pICln (CLNS1A), Oryctolagus cuniculus (Rabbit), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rabbit Methylosome subunit pICln (CLNS1A)

Recombinant Rabbit Methylosome subunit pICln (CLNS1A) — Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Purity >90%.

SKU: BCRECP-002392 Category:
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Product Specifications

Product SkuBCRECP-002392
Product DescriptionRecombinant Rabbit Methylosome subunit pICln (CLNS1A) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-236aa. [Accession | Q28678].
Uniprot No.Q28678
Gene NamesCLNS1A
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-236aa
SpeciesOryctolagus cuniculus (Rabbit)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core).

Biological Context

Subcellular Location: Cytoplasm, cytosol; Nucleus; Cytoplasm, cytoskeleton
Tissue Specificity: Widely distributed but expressed more abundantly in nonpigmented ciliary epithelial cells than in pigmented ones

Product Specifications

Recombinant Rabbit Methylosome subunit pICln (CLNS1A) is a recombinant protein from Oryctolagus cuniculus (Rabbit), expressed in E.coli, covering amino acids 2-236aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 33.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins (By similarity). Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (By similarity). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (By similarity). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (By similarity). Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (By similarity)

Subcellular Location

Cytoplasm, cytosol; Nucleus; Cytoplasm, cytoskeleton

Tissue Specificity

Widely distributed but expressed more abundantly in nonpigmented ciliary epithelial cells than in pigmented ones

Subunit

Component of the methylosome, a 20S complex containing at least PRMT5/SKB1, WDR77/MEP50 and CLNS1A/pICln. May mediate SNRPD1 and SNRPD3 methylation. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP. Interacts with LSM10 and LSM11

Gene: CLNS1A  |  Organism: Oryctolagus cuniculus  |  Synonyms: Chloride channel, nucleotide sensitive 1A; Chloride conductance regulatory protein ICln

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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