Recombinant Rat Carbonic anhydrase 2 (Ca2), Rattus norvegicus (Rat), Yeast, C-terminal 6xHis-tagged, 30.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rat Carbonic anhydrase 2 (Ca2)

Recombinant Rat Carbonic anhydrase 2 (Ca2) — Catalyzes the reversible hydration of carbon dioxide. Purity >90%.

SKU: BCRECP-002279 Category:
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Product Specifications

Product SkuBCRECP-002279
Product DescriptionRecombinant Rat Carbonic anhydrase 2 (Ca2) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 2-260aa. [Accession | P27139].
Uniprot No.P27139
Gene NamesCa2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-260aa
SpeciesRattus norvegicus (Rat)
Tag InfoC-terminal 6xHis-tagged
Molecular weight30.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Catalyzes the reversible hydration of carbon dioxide. Can also hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.

Biological Context

Subcellular Location: Cytoplasm; Cell membrane

Product Specifications

Recombinant Rat Carbonic anhydrase 2 (Ca2) is a recombinant protein from Rattus norvegicus (Rat), expressed in Yeast, covering amino acids 2-260aa, with C-terminal 6xHis-tagged tag, molecular weight 30.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the reversible hydration of carbon dioxide (By similarity). Can also hydrate cyanamide to urea (By similarity). Involved in the regulation of fluid secretion into the anterior chamber of the eye (By similarity). Essential for bone resorption and osteoclast differentiation (PubMed:9665810). Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption (By similarity). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (By similarity)

Subcellular Location

Cytoplasm; Cell membrane

Subunit

Interacts with SLC4A4 and SLC26A6. Interaction with SLC4A7 regulates SLC4A7 transporter activity (By similarity)

Gene: Ca2  |  Organism: Rattus norvegicus  |  Synonyms: Carbonate dehydratase II; Carbonic anhydrase II; Cyanamide hydratase CA2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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