Recombinant Rat CMRF35-like molecule 1 (Cd300lf), partial, Rattus norvegicus (Rat), E.coli, N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged, 38.3kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Rat CMRF35-like molecule 1 (Cd300lf), partial

Recombinant Rat CMRF35-like molecule 1 (Cd300lf), partial — Acts as an inhibitory receptor for myeloid cells and mast cells. Purity >90%.

SKU: BCRECP-002321 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-002321
Product DescriptionRecombinant Rat CMRF35-like molecule 1 (Cd300lf) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 19-181aa. [Accession | Q566E6].
Uniprot No.Q566E6
Gene NamesCd300lf
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region19-181aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight38.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Rat CMRF35-like molecule 1 (Cd300lf), partial is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 19-181aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 38.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands. May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2. Inhibits osteoclast formation. Induces macrophage cell death upon engagement

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with PTPN6/SHP-1 in a tyrosine phosphorylation dependent manner. Interacts with IL4R

Gene: Cd300lf  |  Organism: Rattus norvegicus  |  Synonyms: CD300 antigen-like family member F

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top