Recombinant Rat COMM domain-containing protein 5 (Commd5), Rattus norvegicus (Rat), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 31.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat COMM domain-containing protein 5 (Commd5)

Recombinant Rat COMM domain-containing protein 5 (Commd5) — Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. Purity >85%.

SKU: BCRECP-002414 Category:
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Product Specifications

Product SkuBCRECP-002414
Product DescriptionRecombinant Rat COMM domain-containing protein 5 (Commd5) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-224aa. [Accession | Q9ERR2].
Uniprot No.Q9ERR2
Gene NamesCommd5
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-224aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight31.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. Negatively regulates cell proliferation.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Expressed in the zona fasciculata and medulla of the adrenal gland; expressed in kidney proximal tubules. Basal expression is higher in hypertensive than in normotensive animals

Product Specifications

Recombinant Rat COMM domain-containing protein 5 (Commd5) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 2-224aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 31.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex (By similarity). May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes (By similarity). Negatively regulates cell proliferation (PubMed:12620924). Negatively regulates cell cycle G2/M phase transition probably by transactivating p21/CDKN1A through the p53/TP53-independent signaling pathway (PubMed:12620924). Involved in kidney proximal tubule morphogenesis (PubMed:24515317). Down-regulates activation of NF-kappa-B (By similarity)

Subcellular Location

Nucleus

Tissue Specificity

Expressed in the zona fasciculata and medulla of the adrenal gland; expressed in kidney proximal tubules. Basal expression is higher in hypertensive than in normotensive animals

Subunit

Component of the commander complex consisting of the CCC subcomplex and the retriever subcomplex (By similarity). Component of the CCC (COMMD/CCDC22/CCDC93) subcomplex consisting of COMMD1, COMMD2, COMMD3, COMMD4, COMMD5, COMMD6, COMMD7, COMMD8, COMMD9, COMMD10, CCDC22 and CCDC93; within the complex forms a heterodimer with COMMD10 (By similarity). Interacts (via COMM domain) with COMMD1 (via COMM domain). Interacts with RELA, RELB, NFKB1/p105 (By similarity). Interacts with CCDC22, CCDC93, SCNN1B, CUL2, CUL3, CUL4A, CUL4B, CUL7 (By similarity)

Gene: Commd5  |  Organism: Rattus norvegicus  |  Synonyms: Hypertension-related calcium-regulated gene protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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