Recombinant Rat E3 ubiquitin-protein ligase TRIM63 (Trim63), Rattus norvegicus (Rat), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 47.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Rat E3 ubiquitin-protein ligase TRIM63 (Trim63)

Recombinant Rat E3 ubiquitin-protein ligase TRIM63 (Trim63) — E3 ubiquitin ligase. Purity >90%.

SKU: BCRECP-004621 Categories: ,
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Product Specifications

Product SkuBCRECP-004621
Product DescriptionRecombinant Rat E3 ubiquitin-protein ligase TRIM63 (Trim63) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-351aa. [Accession | Q91Z63].
Uniprot No.Q91Z63
Gene NamesTrim63
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-351aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight47.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Cytoplasm, myofibril, sarcomere, M line; Cytoplasm, myofibril, sarcomere, Z line
Tissue Specificity: Muscle specific. Selectively expressed in heart and skeletal muscle
Pathway: Protein modification; protein ubiquitination

Product Specifications

Recombinant Rat E3 ubiquitin-protein ligase TRIM63 (Trim63) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 1-351aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 47.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells

Subcellular Location

Cytoplasm; Nucleus; Cytoplasm, myofibril, sarcomere, M line; Cytoplasm, myofibril, sarcomere, Z line

Tissue Specificity

Muscle specific. Selectively expressed in heart and skeletal muscle

Subunit

Homodimer. Homooligomer and heterooligomer. Interacts with SUMO2, titin/TTN and GMEB1. Interacts with TRIM54 and probably with TRIM55 and TNNI3. Forms a ternary complex with RACK1 and PRKCE. Interacts with CKM (By similarity)

Pathway

Protein modification; protein ubiquitination

Gene: Trim63  |  Organism: Rattus norvegicus  |  Synonyms: Muscle-specific RING finger protein 1; RING-type E3 ubiquitin transferase TRIM63; Tripartite motif-containing protein 63
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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