Recombinant Rat E3 ubiquitin-protein ligase TRIM63 (Trim63)

Function

E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells

Subcellular Location

Cytoplasm; Nucleus; Cytoplasm, myofibril, sarcomere, M line; Cytoplasm, myofibril, sarcomere, Z line

Tissue Specificity

Muscle specific. Selectively expressed in heart and skeletal muscle

Subunit

Homodimer. Homooligomer and heterooligomer. Interacts with SUMO2, titin/TTN and GMEB1. Interacts with TRIM54 and probably with TRIM55 and TNNI3. Forms a ternary complex with RACK1 and PRKCE. Interacts with CKM (By similarity)

Pathway

Protein modification; protein ubiquitination

• Bodine S.C. et al. Identification of ubiquitin ligases required for skeletal muscle atrophy. — Science (2001)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• McElhinny A.S. et al. Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. — J. Cell Biol. (2002)
• Dehoux M.J.M. et al. Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection. — FEBS Lett. (2003)
• Wray C.J. et al. Sepsis upregulates the gene expression of multiple ubiquitin ligases in skeletal muscle. — Int. J. Biochem. Cell Biol. (2003)
• Lecker S.H. et al. Multiple types of skeletal muscle atrophy involve a common program of changes in gene expression. — FASEB J. (2004)
• Arya R. et al. Muscle ring finger protein-1 inhibits PKC-epsilon activation and prevents cardiomyocyte hypertrophy. — J. Cell Biol. (2004)
• Hong-Brown L.Q. et al. Indinavir alters regulators of protein anabolism and catabolism in skeletal muscle. — Am. J. Physiol. (2005)