Recombinant Rat Galectin-3 (Lgals3)

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity). Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (PubMed:11121438). When secreted, interacts with NK cell-activating receptor NCR3/NKp30 acting as an inhibitory ligand which antagonizes NK cell attack (By similarity)

Subcellular Location

Cytoplasm; Nucleus; Secreted

Subunit

Probably forms homo- or heterodimers. Interacts with DMBT1 (PubMed:11121438). Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4 (By similarity). Interacts with LGALS3BP, LYPD3, ZFTRAF1 and UACA (PubMed:15729693). Interacts with TRIM16; this interaction mediates autophagy of damage endomembranes (By similarity). Interacts with and inhibits by binding NCR3/NKp30 (By similarity)

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• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
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• Liu F.-T. et al. Identification of an IgE-binding protein by molecular cloning. — Proc. Natl. Acad. Sci. U.S.A. (1985)
• Lubec G. et al. PubMed: CI-EJHB3PREPF1GJ (NOV-)
• Herrmann J. et al. Primary structure of the soluble lactose binding lectin L-29 from rat and dog and interaction of its non-collagenous proline-, glycine-, tyrosine-rich sequence with bacterial and tissue collagenase. — J. Biol. Chem. (1993)
• Hikita C. et al. Induction of terminal differentiation in epithelial cells requires polymerization of hensin by galectin 3. — J. Cell Biol. (2000)
• Paret C. et al. Ly6 family member C4.4A binds laminins 1 and 5, associates with galectin-3 and supports cell migration. — Int. J. Cancer (2005)